| Production of L-asparaginase by bacterial and fungal isolates from Egyptian sources using submerged fermentation |
| Paper ID : 1025-ICBAS2024-FULL (R3) |
| Authors |
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Khaled Amr Hassan *1, Yasmin Mostafa Atia2, Fatma Ibrahim Abou-Elazm1, Asmaa Abdelaal3, Mona Tawfik Kashef4 1Department of Microbiology and Immunology, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, 6th of October City, Egypt 2Lecturer of Pharmacology and Experimental Therapeutics, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt 3Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt 4Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt |
| Abstract |
| Aim: L-asparaginase (L-ASNase) is a widely used antineoplastic agent, especially in treating acute lymphoblastic leukemia, besides its use in food processing. Microbes are very efficient producers of the enzyme. Here, we screened microorganisms isolated from different Egyptian sources for L-asparaginase production, by submerged fermentation. Methods: The samples collected for microbial isolation were from soil (n =10), collected from different locations in Giza, Egypt, honey (n =7), purchased from commercial markets, and rotten fruits and vegetables (n = 6). Microbial isolates were obtained using the streak plate method. The isolated bacteria and fungi were screened for asparaginase activity using modified M9 media and modified Czapex dox media, respectively, containing asparagine as a substrate, and phenol red as an indicator. The asparaginase-positive isolates were assayed for asparaginase activity. Results: Among 55 bacterial isolates, ten were asparaginase-positive with a specific activity between 0.096 ± 0.0078 to 0.43 ± 0.0289 U/mg; the maximum specific activity was recorded with HB1 isolate from the black seed honey sample. Furthermore, out of the 37 fungal isolates, 15 had positive L-asparaginase production. The specific activity recorded for fungal isolates ranged between 0.21 ± 0.09698 to 1.385 ± 0.2723 U/mg, with the isolate SL8, from a soil sample, having a maximum specific activity. Conclusion: Environmental and honey isolates are promising sources of L-asparaginase enzyme production by submerged fermentation. Fungal isolates produce more potent enzymes than bacteria. Further optimization is required to enhance the enzyme activity for efficient production. |
| Keywords |
| Acute lymphoblastic leukemia, Bacteria, Fungi, L-asparaginase, Submerged fermentation |
| Status: Accepted (Poster Presentation) |